AFB DNA (TB-PCR) Detection by Real Time PCR , Reflex to Rifampicin resistance by Ultra CBNAAT (If - MTB Positive) - Body Fluids

AFB DNA (TB‑PCR) by real‑time PCR is a molecular assay designed to detect Mycobacterium tuberculosis complex DNA directly from body fluids (for example CSF, pleural, peritoneal, synovial fluids). The test identifies MTB DNA rapidly and sensitively, with an automatic reflex to Ultra CBNAAT (CB‑NAAT) for rifampicin resistance testing if the PCR result is MTB positive, enabling prompt evaluation of potential drug resistance.

This combination test is particularly useful for suspected extrapulmonary or paucibacillary TB where microscopy is often negative, and for cases requiring rapid diagnostic information to guide clinical decisions. Home sample collection is available across Dubai and the UAE, with results and interpretation provided by accredited laboratories; note that a negative molecular result does not completely exclude TB and culture or additional testing may be recommended by your clinician.

• Mycobacterium tuberculosis complex (MTB) DNA — TB‑PCR result
• Cycle threshold (Ct) / semi‑quantitative PCR signal
• Internal control / assay validity
• Rifampicin resistance (Detected / Not detected) by Ultra CBNAAT (reflex)

• Clinical suspicion of extrapulmonary TB (e.g., pleural, CSF, peritoneal, synovial fluids)
• Paucibacillary or smear‑negative suspected TB cases
• Rapid assessment needed for treatment decisions or isolation measures
• Suspected drug‑resistant TB or history of prior TB treatment
• Neurological signs suggestive of tuberculous meningitis

• Rapid molecular detection of MTB DNA compared with conventional culture
• Reflex Ultra CBNAAT testing for rifampicin resistance when MTB is detected
• Improved sensitivity for paucibacillary and extrapulmonary samples
• Convenient at‑home sample collection across Dubai and the UAE
• Actionable results to guide early treatment and public‑health decisions

• Sterile collection of appropriate body fluid (CSF, pleural, peritoneal, synovial, or other body fluids)
• At‑home collection performed by trained phlebotomist/technician; follow sample labelling instructions
• No fasting required; inform team of current or recent TB treatment and antibiotics
• Ensure prompt transport to laboratory per instructions to preserve nucleic acid integrity