
HIV I & II Western Blot Test
Author Details
Written By
Dr. Arjun Nair
MD (Internal Medicine)
Supervised By
Dr. Ananya Rao
MD (Microbiology)





HIV I & II Western Blot Test
Confirmatory HIV I & II Western Blot testing with professional at-home sample collection in Dubai, providing accurate, private results and reliable clinical confirmation.
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Know More About HIV I & II Western Blot Test
The HIV I & II Western Blot test is a laboratory confirmatory assay that detects antibodies directed against specific HIV viral proteins, using band-pattern recognition to distinguish HIV‑1 and HIV‑2 immune responses. It analyses serum or plasma for antibodies to structural and envelope proteins (for example p24, gp41, gp120/160 for HIV‑1 and characteristic gp36/gp105 bands for HIV‑2) and interprets the presence or absence of these bands to confirm or exclude infection. This method offers high specificity and is used to resolve reactive or indeterminate results from initial screening tests.
This service is provided with professional at‑home venous blood collection in Dubai, ensuring confidentiality and convenient sample handling to accredited UAE diagnostic laboratories. The Western Blot is recommended following a reactive screening test (such as a 4th‑generation antigen/antibody test) or when clinical suspicion persists despite negative screening during the window period; results support clinical decision‑making, linkage to care, and public‑health reporting as required under local regulations. Interpretation should be performed by qualified laboratory physicians and combined with clinical assessment and, when appropriate, nucleic acid testing for early infection.
Parameters Measured
- HIV‑1 p24
- HIV‑1 gp41
- HIV‑1 gp120/gp160
- HIV‑2 gp36
- HIV‑2 gp105
- Internal control band / assay validity
When to Test it?
- After a reactive HIV screening test (ELISA/4th‑gen) for confirmatory diagnosis
- When initial screening results are indeterminate or conflicting
- Following a credible high‑risk exposure once the window period has elapsed
- When clinical signs or opportunistic infections suggest possible HIV infection
- To differentiate between HIV‑1 and HIV‑2 in cases with suspected non‑1 infections
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Benefits Includes:
- High specificity to confirm or rule out HIV infection after screening
- Ability to differentiate HIV‑1 versus HIV‑2 antibody patterns
- Professional, private at‑home blood collection across Dubai
- Results suitable for clinical confirmation and linkage to treatment services
- Accredited laboratory analysis with expert interpretation and reporting
Doctor Recommended Checkups
Test requirements:
- Venous blood sample collected by a trained phlebotomist (at home or clinic)
- No fasting required prior to sample collection
- Previous reactive screening test or clinician referral is recommended
- Valid ID and informed consent required for testing and result release in UAE
Author Details
Written By
Dr. Arjun Nair
MD (Internal Medicine)
Supervised By
Dr. Ananya Rao
MD (Microbiology)

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