Malaria By real time PCR

Malaria by Real Time PCR is a molecular diagnostic test that detects and identifies Plasmodium parasite DNA in a patient’s blood using real-time polymerase chain reaction amplification. The assay targets species-specific genetic sequences to provide highly sensitive and specific detection, capable of identifying low-level parasitaemia and mixed infections that may be missed by microscopy or rapid diagnostic tests. Samples can be collected at home by trained phlebotomists and are analysed in Dubai‑certified laboratories to ensure reliable results and compliance with UAE regulatory standards.

This test is recommended for patients with fever after travel to endemic regions, cases with persistent or recurrent fever despite negative smears or rapid tests, and when accurate species identification is required to guide targeted therapy. Results are typically available within 24–48 hours depending on laboratory workflow; interpretation should be performed in conjunction with clinical findings and history (travel, prophylaxis, recent antimalarial treatment). Note that PCR detects parasite DNA and may not distinguish between viable and recently cleared infections, so clinicians may combine PCR findings with clinical assessment and other laboratory tests for treatment decisions.

• Plasmodium spp. DNA (genus-level)
• Plasmodium falciparum DNA
• Plasmodium vivax DNA
• Plasmodium malariae DNA
• Plasmodium ovale DNA
• Plasmodium knowlesi DNA

• Fever after travel to malaria‑endemic areas
• Persistent or recurrent fever with negative microscopy/RDT
• Suspected mixed or low‑level parasitaemia
• Before or during treatment when species confirmation is needed
• Investigation of treatment failure or recrudescence

• High sensitivity for low parasite loads
• Accurate species identification to guide therapy
• Detection of mixed Plasmodium infections
• Home sample collection with testing in Dubai‑certified labs
• Fast turnaround suitable for timely clinical decisions

• At‑home venous or capillary blood collection by trained phlebotomist
• No fasting required prior to sample collection
• Provide recent travel history, symptoms onset, and current medications
• Valid Emirates ID or passport details for lab processing